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pe cyanine 7 cd206  (Elabscience Biotechnology)


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    Elabscience Biotechnology pe cyanine 7 cd206
    Pe Cyanine 7 Cd206, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+cyanine+7+cd206/PE%2FCyanine7+Anti-Mouse+CD206%2FMMR+Antibody/pm41996989-66-30-35
    Average 94 stars, based on 16 article reviews
    pe cyanine 7 cd206 - by Bioz Stars, 2026-10
    94/100 stars

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    Centrifugation:

    Article Title: Role of circSRSF1 in apoptosis, inflammation, and macrophage polarization during acute respiratory distress syndrome.
    Article Snippet: Acute respiratory distress syndrome (ARDS) is a common respiratory disorder in surgical intensive care units, and its unique pathological features and pathogenesis pose significant health risks to patients.. Although circular RNAs have been implicated in various disease processes, their biological functions in ARDS remain largely unexplored.. We aimed to investigate the role of circSRSF1 in lipopolysaccharide (LPS)-induced lung injury, thereby providing a basis for biomarker screening and the identification of potential therapeutic targets for ARDS.



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    Both in vitro and in vivo, MPB-3BP@CM NPs-induced macrophage polarization and macrophage phagocytosis. The flow cytometric analysis was performed to quantify both M1-like macrophages (CD86+) ( a ) and M2-like macrophages (CD206+) ( b ) were quantified in RAW264.7 gating on F4/80 + CD11b+ cells (I: PBS, II: CM NPs, III: MPB NPs + laser, IV: 3BP, V: MPB-3BP@CM NPs, VI: MPB-3BP@CM NPs + laser). ELISA measurement of the secretion of IL-6 ( c ), IL-12 ( d ), IL-10 ( e ), and TGF-β ( f ) in different treatment groups. g Quantification analysis reveals that MPB-3BP@CM NPs-mediated CD47 blockade enhances phagocytosis of HCT116-EGPF cancer cells by BMDMs in vitro. h Quantification analysis of the phagocytosis of HCT116-EGPF cancer cells by BMDMs after CD47 blockade with varying concentrations of MPB-3BP@CM NPs in vitro. i Schematic illustration showing the timelines of treatment. Quantification analysis of M1-like macrophages (CD86+) ( j ) and M2-like macrophages (CD206+) ( k ) in tumor gating on F4/80 + CD11b+ cells (i: saline, ii: MPB NPs + laser, iii: 3BP, iv: CM NPs, v- MPB-3BP@CT NPs + laser, vi: MPB-3BP@CM NPs, vii: MPB-3BP@CM NPs + laser). Secretion levels of IL-6 ( l ), IL-12 ( m ), IL-10 ( n ), and TGF- β ( o ) in tumor tissues of mice after different treatments. p Representative image and quantitative analysis of immunofluorescence staining of the tumor sections showing infiltrated CD86 + F4/80+ macrophage cells. Scale bar = 50 μm. q Representative flow cytometry plots of CFSE + F4/80 + CD11b+ cells in tumor gating on F4/80 + CD11b+ cells, the phagocytosis efficiency is represented by the percentage of CFSE + F4/80 + CD11b+ cells in total F4/80 + CD11b+ cells. All data were presented as mean ± SD ( n ≥ 3)

    Journal: Signal Transduction and Targeted Therapy

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    Figure Lengend Snippet: Both in vitro and in vivo, MPB-3BP@CM NPs-induced macrophage polarization and macrophage phagocytosis. The flow cytometric analysis was performed to quantify both M1-like macrophages (CD86+) ( a ) and M2-like macrophages (CD206+) ( b ) were quantified in RAW264.7 gating on F4/80 + CD11b+ cells (I: PBS, II: CM NPs, III: MPB NPs + laser, IV: 3BP, V: MPB-3BP@CM NPs, VI: MPB-3BP@CM NPs + laser). ELISA measurement of the secretion of IL-6 ( c ), IL-12 ( d ), IL-10 ( e ), and TGF-β ( f ) in different treatment groups. g Quantification analysis reveals that MPB-3BP@CM NPs-mediated CD47 blockade enhances phagocytosis of HCT116-EGPF cancer cells by BMDMs in vitro. h Quantification analysis of the phagocytosis of HCT116-EGPF cancer cells by BMDMs after CD47 blockade with varying concentrations of MPB-3BP@CM NPs in vitro. i Schematic illustration showing the timelines of treatment. Quantification analysis of M1-like macrophages (CD86+) ( j ) and M2-like macrophages (CD206+) ( k ) in tumor gating on F4/80 + CD11b+ cells (i: saline, ii: MPB NPs + laser, iii: 3BP, iv: CM NPs, v- MPB-3BP@CT NPs + laser, vi: MPB-3BP@CM NPs, vii: MPB-3BP@CM NPs + laser). Secretion levels of IL-6 ( l ), IL-12 ( m ), IL-10 ( n ), and TGF- β ( o ) in tumor tissues of mice after different treatments. p Representative image and quantitative analysis of immunofluorescence staining of the tumor sections showing infiltrated CD86 + F4/80+ macrophage cells. Scale bar = 50 μm. q Representative flow cytometry plots of CFSE + F4/80 + CD11b+ cells in tumor gating on F4/80 + CD11b+ cells, the phagocytosis efficiency is represented by the percentage of CFSE + F4/80 + CD11b+ cells in total F4/80 + CD11b+ cells. All data were presented as mean ± SD ( n ≥ 3)

    Article Snippet: Following 48 h of incubation, RAW264.7 macrophages from the upper chamber were acquired and stained with fluorescence-conjugated anti-mouse CD11b (eBioscience, FITC), CD206 (eBioscience, PE-cyanine 7), CD86 (eBioscience, APC), F4/80 (eBioscience, PE) antibodies.

    Techniques: In Vitro, In Vivo, Enzyme-linked Immunosorbent Assay, Saline, Immunofluorescence, Staining, Flow Cytometry